Composite

Part:BBa_K5250013:Design

Designed by: Ekaterina Tocheva   Group: iGEM24_UZurich   (2024-10-01)


Constitutive promoter, XutR + Pxut, GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal BamHI site found at 1236
    Illegal BamHI site found at 4066
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1507
    Illegal BsaI site found at 2571
    Illegal BsaI site found at 3441
    Illegal BsaI.rc site found at 1459
    Illegal BsaI.rc site found at 1951
    Illegal BsaI.rc site found at 2879
    Illegal BsaI.rc site found at 3889
    Illegal SapI site found at 3870
    Illegal SapI.rc site found at 3065


Design Notes

The sequences are codon optimized.


Source

The sequences for XutR and Pxut were obtained from this paper: https://doi.org/10.1128/aem.00250-20. The rest of the sequences are from the iGEM Parts Registry.

References